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  • Mouse kidney, 16um axial thickness, Alexa Fluor 488 WGA, 60x Plan Apo, NA 1.4
  • Mouse kidney, 16um axial thickness, stained with Alexa Fluor 568 phalloidin (red) and Alexa Fluor 488 WGA (green) 100x Apo Tirf, NA 1.49
  • Mouse kidney, 16um axial thickness, Alexa Fluor 568 phalloidin,  60x Plan Apo, NA 1.4
Mouse kidney, 16um axial thickness, Alexa Fluor 488 WGA, 60x Plan Apo, NA 1.41 Mouse kidney, 16um axial thickness, stained with Alexa Fluor 568 phalloidin (red) and Alexa Fluor 488 WGA (green) 100x Apo Tirf, NA 1.492 Mouse kidney, 16um axial thickness, Alexa Fluor 568 phalloidin,  60x Plan Apo, NA 1.43
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Mouse brain  

Mouse brain confocal performance print

60 μm thick section of an entire mouse brain.
The sample is stained with 4 colours (3 antibodies and DAPI) and has been scanned by TissueFAXS Confocal at 20x magnification factor.
1400 images per each confocal plane and a total of 29400 images for 21 layers z-stack.

Courtesy of TissueGnostics. Acquisitions by courtesy of Janelia Farm Research Campus, Howard Hughes Medical Institute, Virginia, USA.

Mouse kidney tissue

Mouse kidney tissue

 


Mouse kidney tissue - Large field of view


4μm beads acquisition  

4μm beads acquisition - Large field of view

Extended filed of view confocal acquisition of 4μ beads layer through the V2 L-FOV imager. The field of view covered is 25mm. A crop of the beads in the outer regions of the field shows the high quality of the optical imaging.

Daphnia  

Daphnia - Large field of view

Comparison between a standard V2 18mm field of view confocal acquisition and a V2 L-FOV 25mm field of view confocal acquisition of a Daphnia magnified 20x

Stack projection of HeLa cells with Qdot™ Conjugates. Nucleus – Qdot™ 655; Golgi – Qdot™ 585; Microtubules – Qdot™ 525.  

Stack projection of HeLa cells with Qdot™ Conjugates. Nucleus – Qdot™ 655; Golgi – Qdot™ 585; Microtubules – Qdot™ 525.

Quantum Dot Corporation, Hayward, CA

Stack projection of mouse intestine section labeled with Alexa Fluor® 350 WGA, Alexa Fluor® 568 phalloidin, and SYTOX® Green.

Stack projection of mouse intestine section labeled with Alexa Fluor® 350 WGA, Alexa Fluor® 568 phalloidin, and SYTOX® Green.

Molecular Probes/Invitrogen, Eugene, OR

3D reconstruction of imaged skin sample labeled using pan-neuronal marker, protein gene product 9.5 localized with Cy3 and basement membrane marker, type IV collagen with CY2. Endothelial cells are stained using Cy5-Ulex europeaus agglutinin type I.  

3D reconstruction of imaged skin sample labeled using pan-neuronal marker, protein gene product 9.5 localized with Cy3 and basement membrane marker, type IV collagen with CY2. Endothelial cells are stained using Cy5-Ulex europeaus agglutinin type I.

Dr. William R. Kennedy and Gwen Wendelschafer-Crabb, University of Minnesota, Minneapolis, MN

Maximum projection and orthogonal view created from confocal sections through a sea urchin embryo fixed and stained with anti-tubulin (green) and phalloidin (red).

Maximum projection and orthogonal view created from confocal sections through a sea urchin embryo fixed and stained with anti-tubulin (green) and phalloidin (red).

Tubulin (green), Actin (red), (60X 1.4NA) Dr. George von Dassow, Center for Cell Dynamics, Friday Harbor Labs, University of Washington, Friday Harbor, WA

Maximum projection and orthogonal view created from confocal sections through a sea urchin embryo fixed and stained with anti-tubulin (green) and phalloidin (red).

Maximum projection and orthogonal view created from confocal sections through a sea urchin embryo fixed and stained with anti-tubulin (green) and phalloidin (red).

Orthogonal view (XZ)

Time lapse images at a single confocal plane of a sand dollar embryo during mid blastula stage (rhodamine-tubulin and Alexa 488 phalloidin, Molecular Probes, Eugene, Oregon).

Time lapse images at a single confocal plane of a sand dollar embryo during mid blastula stage (rhodamine-tubulin and Alexa 488 phalloidin, Molecular Probes, Eugene, Oregon).

Dr. George von Dassow, Bill Bement, Center for Cell Dynamics, Friday Harbor Labs, University of Washington, Friday Harbor, WA

Time lapse images at a single plane of a Nematode embryo going through the first cell division stage. GFP-tubulin, GFP-histone.

Time lapse images at a single plane of a Nematode embryo going through the first cell division stage. GFP-tubulin, GFP-histone.

Dr. Fabio Piano, NYU, New York, NY

Time lapse images and FRAP kinetics of endoplasmic reticulum in a plant cell stained with the membrane dye 3,3-dihexyloxacarbocyanine iodide (DiO6(3), Molecular Probes, Eugene, Oregon).

Time lapse images and FRAP kinetics of endoplasmic reticulum in a plant cell stained with the membrane dye 3,3-dihexyloxacarbocyanine iodide (DiO6(3), Molecular Probes, Eugene, Oregon).

The FRAP protocol including image capture and analysis was performed using IPLab (Scanalytics, Fairfax, VA) imaging software.

Time lapse recordings at 50 frames per second of calcium sparks in muscle cells loaded with calcium indicator dye Fluo-4, Molecular Probes, Eugene, Oregon.

Time lapse recordings at 50 frames per second of calcium sparks in muscle cells loaded with calcium indicator dye Fluo-4, Molecular Probes, Eugene, Oregon.

The Roper Cascade 512B CCD camera in conjunction with Metamorph® (Molecular Devices, Sunnyvale, CA) imaging software was used to capture the images.

Plant Stems

Plant Stems

Dr. David Carter, UC Riverside, CA

Drosophila Embryo- GFP-Tubulin

Drosophila Embryo- GFP-Tubulin

Dr. Garrett Odell, Friday Harbor, University of Washington, WA

Human Skin, maximum projection CY2-Basement membrane, CY3-Neuron, CY5- Endothelial cell

Human Skin, maximum projection CY2-Basement membrane, CY3-Neuron, CY5- Endothelial cells

Dr. William R. Kennedy and Gwen Wendelschafer-Crabb, University of Minnesota, MN

 

Crestoptics S.p.A.

Via Mattia Battistini, 184/D - 00167 ROMA

Tel. 0039.06.61660508 - FAX 0039.06.61662738

e-mail: info@crestopt.com

 

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