Deep imaging of clarified
mammary gland tissue
Deep imaging of mammary gland tissue clarified by uDISCO technique, an organic solvent-based clearing method.
Here a 3D volume view showing keratine 8 (red) keratine 5 (yellow) and DAPI (blue) taken with CrestOptics X-Light V3.
The use of Silicon immersion objectives, like the Plan Apochromat Lambda S 25XC Sil (Nikon), allows to combine the highest achievable resolution with an outstanding penetration, essential when working with clarified tissies.
Added to the exclusive 25mm FOV of the X-light V3, this setup allows to image a unique volume without the need of stitching.
Max projection of 500um thick 3D acquisition in clarified mammary gland tissue
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