Hippocampal dendritic spines

using spinning disk microscopy

Hippocampal coronal slice (60 µm thickness) from Thy1‐GFP mouse brain was acquired with X-Light V2 Confocal Spinning Disk. In this mouse line, Thy1-expressing neurons show Thy1 labeled with GFP at the cell surface of mature neurons and that makes the sample suitable for high‐resolution imaging.

Here an image stack of 293 Z planes (total volume of 29.3 µm) is shown. In particular, you can appreciate dendritic spines (zoom image), micron-sized protrusions along the dendritic shaft where the majority of excitatory inputs are located. Spines are dynamic structures in shape and numbers, reflecting the plastic nature of synaptic connections, a key aspect of learning and memory. Moreover, alteration in spine density is often correlated with both neurodevelopmental and neurodegenerative diseases. Therefore, imaging dendritic spines allow us to better understand changes occurring in the brain in physiological and pathological conditions.

X-light V2 acquisition

(Plan Apochromat Lambda 100X oil, 1.45 NA)

Maximum intensity projection

Zoom

3D volume reconstruction and movie

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