Imaging a living Zebrafish

Acquisition @ Scuola di Microscopia 2019 – Transgenic zebrafish (4 days old) expressing fluorescent proteins: lyn-TagRFP, neuron cellular membrane marker, and nls-GCaMP6s, calcium sensor in the nucleus.

Z-projection (Maximum Intensity)

Confocal Spinning Disk acquisition

Autoquant 3D Deconvolution (theoretical PSF) and zoomed inset shown

Zoom

Sample courtesy of Marco Dal Maschio

Department of Biomedical Sciences, University of Padua

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