Imaging a living Zebrafish

Acquisition @ Scuola di Microscopia 2019, in collaboration with Crisel Instruments srl

Transgenic Zebrafish (4 days old) expressing fluorescent proteins: lyn-TagRFP, neuron cellular membrane marker, and nls-GCaMP6s, calcium sensor in the nucleus. A maximum intensity Z-projection is shown.

X-Light V2 Confocal Spinning Disk

acquisition

Autoquant 3D Deconvolution (theoretical PSF)

and zoomed inset shown

3D movie of deconvolved image

Sample courtesy of Marco Dal Maschio

Department of Biomedical Sciences, University of Padua

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