NIR imaging in spinning disk confocal
The standard port on the illuminator of X-light V2 and X-light V3 allows to use light sources in the 400-750 nm ranges without any special accessories. Thanks to this specification, it is possible to simultaneously image DAPI and NIR dyes such as Cy7 on the same samples. Such capability is essential for multi-stained specimens with 4 or more channels, where the wide spectral window allows for a better choice of non-overlapping dyes.
Here is shown a mouse brain sample stained with an in situ sequencing kit that can be directly applied to tissue sections.
DAPI and Cy7 stitched image
(X-light V3; 20x objective)
The brain samples were kindly provided by the VIB BioImaging Core in Leuven (Belgium)
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