VCS2019-01-17T15:19:27+00:00

VCS

VCS upgrade X-Light V2 to experience the full 3D
super-resolution of structured illumination microscopy

CONFOCAL UPGRADE

LATERAL SUPER-RESOLUTION

SUPER-RESOLUTION SECTIONING

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Description
VCS (VideoConfocal super-resolution) module is a new system developed as an add-on for the X-light V2.

Using an approach based on super-resolution patents from Pisa CNR (which assume the 2-dimensional scan of the sample with a pinhole pattern and several algorithms application), CrestOptics-VCS enhance 3D resolution of the standard light microscope.

Parallel calculation is implemented exploiting CUDA technology for a very fast processing time.

The full system X-Light V2 + VCS is thought to work in three configurations:

  • Widefield mode
  • Confocal mode
  • Super-resolution mode

CrestOptics has been admitted to the following tender:

“BREVETTI +” SOTTOMISURA 2 “INCENTIVI PER LA VALORIZZAZIONE ECONOMICA DEI BREVETTI”

Public Announcement dated August 3, 2011
Protocol N°5002159 – COD. DOMANDA 3717EC submitted on June 23, 2014
CUP code: C84E15000260008

Project title: “Metodo per la raccolta di immagini in microscospia confocale”
Contract stipulated with Invitalia on  December 23, 2015

Technical Specifications

Software controlled bypass mode for widefield, confocal and super-resolution

Up to 18mm field of view

Lateral resolution ~120nm*

Axial resolution ~300nm*

Highly corrected immersion objectives compatible

  • 60x
  • 100x

Suitable for thick specimen imaging < 100um

Time resolution: ~ 5 sec/frame

Camera specification:

  • 6.5µm pixel size camera
  • sCMOS QE>80%, low readout noise ≤ 1e- strongly recommended
Video Card/CUDA specifications:

Reccomended requirments:

  • Compute capability version: 5.0 or higher
  • GPU Memory: 4 Gb or higher

Link to compute capability versions and video cards CUDA supported:

PC/Workstation RAM 16 Gb or higher

Elaboration speed depends on GPU performances

* Dependant on optics properties, objectve magnification and camera pixel size

VCS/SCSR pixel calibration table+
Camera pixel size6.5 µm11 µm13 µm
Magnification
60x54.2 nm/pixel91.6 nm/pixel108 nm/pixel
100x32.5 nm/pixel55 nm/pixel65 nm/pixel

+Pixel calibration after using Super-Confocal/Super-Resolution VCS algorithm. A 6.5µm
pixel camera must be used to have the required sampling for maximum lateral resolution.

How you see

Comparison of Hela Cells Interphase in standard resolution vs VCS super-resolution

standard resolution

VCS super-resolution

COMPATIBILITY

Compatible Products

Detectors

Photometrics
Andor technology
Hamamatsu
PCO

Microscopes

Nikon
Olympus
Leica
Zeiss

Illuminators

89North – LDI
Excelitas
Lumencor

Software

MetaMorph
Nis-Element
MicroManager
Volocity
VisiView

If you have questions about compatibility,
write us