Ventricle-like structure in human iPSC derived cortical organoid

Human iPSC are able to generate cortical organoids in vitro after a specific differentiation protocol.
Here is shown a ventricle-like structure within a cortical organoid of 50 days in vitro stained for MAP2 (in cyan), Pax6 (in red) and N-Cadherin (in green), which are markers for ventricular and subventricular-like regions.

X-Light V3 Confocal Spinning Disk acquisition (40x objective)

Zoom in (60x objective)

Raw spinning disk

After deconvolution

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