X-Light V3 is the next generation of X-Light spinning disk confocal series.
It relies on the cutting-edge technology, advanced optical design approach and engineering solutions developed by CrestOptics to meet the very high-end specifications required by most of the modern fluorescence microscopy applications.
The spinning disk design together with the careful design of the optical layout enhances sensitivity and image clarity.
The first confocal unit which allows dual camera imaging at the full field of view of 25mm on both cameras.
Paramecium unicellular ciliated protozoa – 3D volume recontruction
Spectral range (confocal/widefield): 400-750nm
Fully automated experiments
Software controlled bypass mode: widefield to confocal
The fastest spinning disk in the market: 15.000 rpm rotation speed, up to kHz range
Choose your disk: 50um pinholes in two different versions based on different pinole spacing
Choose your disk: unique continuous spiral disk makes it faster for high-speed applications
User inspired: customize pinhole size and geometry on request
Exchangeable, synchronized and sealed: the disk box is one of the most versatile features of the new V3. Dust protection and synchronization signals out to the camera make it reliable and faster.
Lateral Resolution (FWHM): ~230 nm (High NA 1.4) diffraction limited
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
High Confocal Quality: this advanced new series brings in a redesigned optical layout optimized to enhance sensitivity and signal-to-noise ratio in any dim and bright conditions
Ultra-large field of view imaging: maximum 25 mm
Dual-camera view: simultaneous dual-camera view up to 25 mm each camera
Software controlled filter cubes: 3 positions for simultaneous dual-color imaging
Illumination uniformity: microlenses based for high uniformity across the full field of view
Illumination FOV: different options to match different camera sizes and enhance efficiency
Illumination shaping: preserve your sample. Motorized aperture to cut illumination in the region surrounding the region of interest
Software controlled filter wheels: 4-positions automated cleanup filter wheel, 3-positions automated dichroic filter wheel, 8-positions automated emission filter wheel
V3 FEATURES INSIGHT
X-LIGHT PINHOLE DISKS
The 50um disk is available in two different versions: standard spacing and larger spacing.
Standard X-Light spinning disk is the excellent compromise between brightness, confocality and image quality. The version with larger spacing has been designed to enhance even more the image quality and sectioning in thicker specimens thanks to the large reduction of crosstalk between pinholes.
50um disk larger spacing
50um standard disk
showing the pinholes spacing in standard version (red) and larger spacing (green). Image acquired using a 4.2 Megapixel sCMOS camera
Microlenses approach makes the illumination field highly homogeneous on large fields of view.
A unique plug-and-play design solution allows you to choose and change the illumination size to match the most common camera formats. If needed it is possible to shrink the illumination on smaller areas.
Smaller areas mean higher power density, higher efficiency and higher speed
Power density increasing at
different illumination FOV size
DUAL COLOR, DUAL IMAGING, STILL WIDER
The first confocal unit which allows dual-camera imaging at the maximum field of view of 25mm…on both cameras.
External regulations allow to easily align the second camera with the first one
SEALED DISK BOX
This unique new feature makes the disk fully isolated and protected from environmental dust and prevents undesired artifacts related to the presence of small dust particles on the disk surface
To manage very high-speed imaging, hundreds of fps, V3 provides as a standard feature a synchronization signal from the spinning disk to the camera.
A good way to make a solid quantitative analysis of very fast dynamics
A software controlled square iris in front of the spinning disk, with variable aperture, can be used to screen light and preserve the surrounding area of the sample out of the region of interest.
Hover on image to see →
If you have questions about compatibility,