X-light V32019-01-17T15:11:12+00:00

X-Light V3

See Brighter,
See Faster,
See More

FULL COMPATIBILITY

HIGH-SECTIONING, HIGH-SPEED

ENHANCED SENSITIVITY AND IMAGE QUALITY

DUAL-CAMERA ULTRA-LARGE FIELD OF VIEW

Product gallery
Description
X-Light V3 is the next generation of X-Light spinning disk confocal series.

It relies on the cutting-edge technology, advanced optical design approach and engineering solutions developed by CrestOptics to meet the very high-end specifications required by most of the modern fluorescence microscopy applications.

The spinning disk design together with the careful design of the optical layout enhances sensitivity and image clarity.

The first confocal unit which allows dual camera imaging at the full field of view of 25mm on both cameras.

Video

Paramecium unicellular ciliated protozoa – 3D volume recontruction

Technical Features

Spectral range (confocal/widefield): 400-750nm

Fully automated experiments

Software controlled bypass mode: widefield to confocal

The fastest spinning disk in the market: 15.000 rpm rotation speed, up to kHz range

Choose your disk: 50um pinholes in two different versions based on different pinole spacing

Choose your disk: unique continuous spiral disk makes it faster for high-speed applications

User inspired: customize pinhole size and geometry on request

Exchangeable, synchronized and sealed: the disk box is one of the most versatile features of the new V3. Dust protection and synchronization signals out to the camera make it reliable and faster.

Lateral Resolution (FWHM): ~230 nm (High NA 1.4) diffraction limited

Axial Resolution (FWHM): ~600 nm (High NA 1.4)

High Confocal Quality: this advanced new series brings in a redesigned optical layout optimized to enhance sensitivity and signal-to-noise ratio in any dim and bright conditions

Ultra-large field of view imaging: maximum 25 mm

Dual-camera view: simultaneous dual-camera view up to 25 mm each camera

Software controlled filter cubes: 3 positions for simultaneous dual-color imaging

Illumination uniformity: microlenses based for high uniformity across the full field of view

Illumination FOV: different options to match different camera sizes and enhance efficiency

Illumination shaping: preserve your sample. Motorized aperture to cut illumination in the region surrounding the region of interest

Software controlled filter wheels: 4-positions automated cleanup filter wheel, 3-positions automated dichroic filter wheel, 8-positions automated emission filter wheel

How you see

V3 FEATURES INSIGHT

X-LIGHT PINHOLE DISKS

The 50um disk is available in two different versions: standard spacing and larger spacing.

Standard X-Light spinning disk is the excellent compromise between brightness, confocality and image quality. The version with larger spacing has been designed to enhance even more the image quality and sectioning in thicker specimens thanks to the large reduction of crosstalk between pinholes.

50um disk larger spacing

50um standard disk

OVERLAY IMAGE

showing the pinholes spacing in standard version (red) and larger spacing (green). Image acquired using a 4.2 Megapixel sCMOS camera

ILLUMINATION

Microlenses approach makes the illumination field highly homogeneous on large fields of view.

A unique plug-and-play design solution allows you to choose and change the illumination size to match the most common camera formats. If needed it is possible to shrink the illumination on smaller areas.

Smaller areas mean higher power density, higher efficiency and higher speed

Power density increasing at
different illumination FOV size

DUAL COLOR, DUAL IMAGING, STILL WIDER

The first confocal unit which allows dual-camera imaging at the maximum field of view of 25mm…on both cameras.

External regulations allow to easily align the second camera with the first one

SEALED DISK BOX

This unique new feature makes the disk fully isolated and protected from environmental dust and prevents undesired artifacts related to the presence of small dust particles on the disk surface

DISK SYNCHRONIZATION

To manage very high-speed imaging, hundreds of fps, V3 provides as a standard feature a synchronization signal from the spinning disk to the camera.

A good way to make a solid quantitative analysis of very fast dynamics

ILLUMINATION SHAPING

A software controlled square iris in front of the spinning disk, with variable aperture, can be used to screen light and preserve the surrounding area of the sample out of the region of interest.

Hover on image to see →

COMPATIBILITY

Compatible Products

Detectors

Photometrics
Andor technology
Hamamatsu
PCO

Microscopes

Nikon
Olympus
Leica
Zeiss

Illuminators

89North – LDI
Excelitas
Lumencor

Software

MetaMorph
Nis-Element
MicroManager
Volocity
VisiView

If you have questions about compatibility,
write us